Anti-UBXN8 Antibody Picoband™

Product Name

Anti-UBXN8 Antibody Picoband™

View all UBXN8 Antibodies

SKU/Catalog Number

A13427-1

Size

100 µg/vial

Form

Lyophilized

Description

Boster Bio Anti-UBXN8 Antibody Picoband™ catalog # A13427-1. Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-UBXN8 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A13427-1)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human UBXN8 recombinant protein (Position: N149-N270).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

A13427-1 is reactive to UBXN8 in Human

Applications

A13427-1 is guaranteed for ELISA, Flow Cytometry, IHC, WB Boster Guarantee

Observed Molecular Weight

36 kDa

Calculated molecular weight

30.541kDa

Background of UBXN8

p97 or VCP (valosin-containing protein) is a versatile ATPase complex, and many cofactors are required for the p97 functional diversity. This gene encodes one of the p97 cofactors. This cofactor is a transmembrane protein and localized in the endoplasmic reticulum (ER) membrane. It tethers p97 to the ER membrane via its UBX domain. The association of this cofactor with p97 facilitates efficient ER-associated degradation of misfolded proteins. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 µg/ml, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 µg/ml, Human
Flow Cytometry, 1-3 µg/1x10⁶ cells, Human
Direct ELISA, 0.1-0.5 µg/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of UBXN8 using anti-UBXN8 antibody (A13427-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HEL whole cell lysates,
Lane 2: human K562 whole cell lysates,
Lane 3: human HepG2 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-UBXN8 antigen affinity purified polyclonal antibody (Catalog # A13427-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for UBXN8 at approximately 36 kDa. The expected band size for UBXN8 is at 31 kDa.

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Figure 2. IHC analysis of UBXN8 using anti-UBXN8 antibody (A13427-1).
UBXN8 was detected in a paraffin-embedded section of human testicular seminoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-UBXN8 Antibody (A13427-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

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Figure 3. Flow Cytometry analysis of RT4 cells using anti-UBXN8 antibody (A13427-1).
Overlay histogram showing RT4 cells stained with A13427-1 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-UBXN8 Antibody (A13427-1, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

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Figure 4. Flow Cytometry analysis of U937 cells using anti-UBXN8 antibody (A13427-1).
Overlay histogram showing U937 cells stained with A13427-1 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-UBXN8 Antibody (A13427-1, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Gene/Protein Information For UBXN8 (Source: Uniprot.org, NCBI)

Alternative Names

D8S2298EUBXD6; Rep-8 protein; REP8UBX domain-containing protein 6; Reproduction 8 protein; Reproduction/chromosome 8; UBX domain containing 6; UBX domain protein 8; UBX domain-containing protein 8 UBXN8 D8S2298E, REP8, UBXD6 UBX domain protein 8 UBX domain-containing protein 8|Reproduction/chromosome 8|UBX domain-containing protein 6|rep-8 protein|reproduction 8 protein

For more info on UBXN8, check out the UBXN8 Infographic

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In this infographic, you will see the following information for UBXN8: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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